Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Degradomics is a subfield of biology that encompasses all genomic and proteomic approaches dedicated to the study of proteases, their inhibitors, and their substrates on a system-wide scale. This includes the analysis of the protease and protease substrate repertoire, also called the 'protease degradome'. The extent of these degradomes can span cellular, tissue, and whole-organism scales. Proteases initiate, regulate, and terminate many important cellular functions through highly specific and limited substrate cleavage. This mechanism, called proteolytic processing, enables precise cellular control of several biological processes, including DNA replication, cell cycle progression, cell proliferation, wound healing, immunity, angiogenesis, and apoptosis. The hierarchical importance of proteases in the system, a key point in drug development is influenced by protease specific activity, redundancy, expression levels, spatiotemporal distribution, zymogen activation, protease turnover, and inhibitory properties. Therefore, a tissue-wide degradomics approach (using genomic and proteomics techniques) is required to characterize members of Ca. termed 'substrate degradomes' for each protease. Protease profiling using a DNA microarray chip provides a general view of the protease transcriptome, whereas messenger RNA expression levels are not predictive of protease protein abundance or activity associated with protease-specific and protease-active protein chips.
Published Date: 2023-03-21; Received Date: 2022-12-29