Biochemistry & Pharmacology: Open Access
Open Access

Abstract

A Novel Application of Wutou (Aconitum carmichaeli) and Banxia (Pinellia ternat) Aqueous Extract on Wound Healing of Rats

Xia Xichao, Liu Hongyang, Wang Weina, Liu Fei, Hu Qingfu, Liang Guina, Zhang Dong and Liu Rongzhi

Objective: The purpose of current study was to investigate effects of application of Wutou and Banxia aqueous extract in the wound rats.

Methods: Rats were fulfilled a surgical lesion with a 2.0 cm resecting tissue in the dorsal fascia. Following, animals were divided into 3 groups, including model group, control group treated with 1 mg/mL of Yunnan Baiyao, and Wutoubanxia group administrated 1 mg/mL of Wutou and Banxia extract. Wound contractions in day 0, 3, 7, 11 were calculated by an image analyzer. The histological analysis was detected using hematoxilin and eosin. The levels of tumor necrosis factor α (TNF-α), interleukin-2 (IL-2), transforming growth factor-β1 (TGF-β1), and basic Fibroblast Growth Factor (bFGF) transcripts in the wound tissue were determined by real-time quantitative PCR.

Results: Compared with the control group, rats in the model group showed poor re-modeling and re-epithelization characterized by a significant decrease of neovascularization, epithelialization and fibroblast. Furthermore, the expression levels of TNF-α, IL-2 were significantly increased, and TGF-β1 and bFGF significantly decreased in the model group in contrasted with that in the control group. By contrast, the treatment of Wutoubanxia extract reversed the above-mentioned conditions caused by wound.

Conclusion: The results suggest that administration of Wutou and Banxia extract has a promoting role in wound healing of rats possibly through enhancing anti-inflammatory ability and inducing fibroblast formation.