Journal of Chromatography & Separation Techniques
Open Access

Abstract

A Novel Fingerprinting Method for Glycosides in Nakshatra Plants by HPTLC

Lokesh T, Dileep KK, Gayathri S, Begum MS, Rameshwar RK, Rajasekhar RB and Varadacharyulu N.CH

Nakshatra plants are medicinally important and used from olden days onwards to treat various diseases. In the present study, a HPTLC method was developed for separation of glycosides in plants. The powdered drug of Nakshatra plant was extracted with ethanol and identification was done chemically by Molisch's, Fehling's, Bial's test and separation of glycosides was performed densitometrically at λ=254-700 nm using as a mobile phase, ethyl acetate: methanol: water (20:2.5:2.5). The results of qualitative analysis revealed the presence of carbohydrates, reducing sugars and pentose. HPTLC method achieved a good separation of glycosides at 254 nm, 304 nm, 354 nm, 404 nm with recorded peak areas and Rf values. The comparative common compound of sample glycosides was also done.