Annals and Essences of Dentistry
Open Access

Abstract

Activity of the Antioxidant Enzymes and RUNX2 Expression on Porous Titanium Alloy Scaffolds

Lais Morandini Carvalho*, Luana Marotta Reis de Vasconcellos, Elis Andrade Lima Zutin, Elisa Mattias Sartori, Daniela Baccelli Silveira Mendonca, Gustavo Mendonca and Yasmin Rodarte Carvalho

Studies have been directed towards the production of new titanium alloys, aiming replacement of TiAlV alloy in the future. Therefore, many mechanisms related to biocompatibility and chemical characteristics have been studied in the field of implantology, but until now, enzyme defenses against oxidative stress have been underexplored. The objective of this study was to evaluate the activity of the antioxidant enzymes Superoxide Dismutase (SOD) and Catalase (CAT) in porous scaffolds of Ti-6 Aluminium-4 Vanadium, Ti-35 Niobium and Ti-35 Niobium-7 Zirconium-5 Tantalum. Porous titanium alloy scaffolds were prepared by powder metallurgy. Mesenchymal stem cells from mouse femur were used. After 24 hours the cells plated under the scaffolds were analyzed by Scanning Electron Microscopy (SEM). The antioxidant enzymes activity was measured after 72 hours of cell plating and after 3,7 and 14-days, cells were collected to evaluate RUNX2 expression by real-time PCR. The SEM images showed the presence of interconnected pores and growth, adhesion and cell spread in the 3 scaffolds. Our results for SOD and CAT were shown to be better for the TiNbZrTa and TiAlV scaffolds. The TiNb scaffold presented less activity for both enzymes. Despite differences between the scaffolds, there were not any statistical differences (p>0.05). RUNX2 presented high expression for TiNbZrTa and TiAlV at day 7 and presented statistical differences just for TiNbZrTa from the control group (TiAlV day 3). At day 14, all scaffolds showed expression above the baseline and exhibited statistical differences from the control group. It was possible to conclude that, as regards antioxidant enzymes, we are not able to confirm any differences which would justify the replacement of TiAlV, but gene expression showed a better capacity for mesenchymal stem cell differentiation in TiNbZrTa, making it a good choice for biomedical applications.

Published Date: 2024-03-26; Received Date: 2019-09-06