International Journal of Advancements in Technology
Open Access

Abstract

Amino Coated Gold Nanorods Based Amperometric Glucose Detection

Shikha Sharma, Navendu Goswami, Nidhi Gupta and Sudha Srivastava

Citrate capped gold nanoseeds of uniform size and shape were prepared and used for CTAB-assisted growth of gold nanorods. Further, shape and size dependent hydrodynamic sedimentation behavior of nanostructure is exploited for separation of gold nanorods from mixture containing particles of different shape and size. The surface functionalization of gold nanorods with amino acids allows its efficient interaction with the enzyme and makes it a promising platform for applications including medical diagnostics and sensing devices. Further, the biosensing efficiency of the glucose biosensor fabricated by immobilizing glucose oxidase (GOx) onto gold nanorods was investigated. Under optimum conditions, the fabricated biosensor showed low detection limit, excellent storage stability and high sensitivity. The biosensor has a wide linear range for the detection of glucose from 5mM to 40mM with 6 s response time. The retained activity of glucose oxidase ensures the biocompatibility of amino coated gold nanorods and shows that it can be extended to other enzymes as well for development of novel biosensors.