Journal of Food: Microbiology, Safety & Hygiene
Open Access

Abstract

An Evaluation on the Recovery of Staphylococcus aureus in CHROMagar Versus Conventional Selective Agar in Food Sample Analysis

Lei Benitez*

In this study, we tried to evaluated the performance criteria’s of CHROMagar^TM (CHROMagar – Paris FRANCE) in comparison with the conventional standard Baird Parker Agar as per the verification protocols. The objective of the study is to adopt the CHROMagar in the enumeration of Staphylococcus aureus in food samples which requires a lesser incubation time in comparison with standards methods which in turn reduces the turnaround time of the test. Performance criteria’s such as Precision, Accuracy, Specificity sensitivity and selectivity were assessed. CHROMagar is highly effective for the isolation and presumptive identification of S. aureus from food samples and that it compares favourably with conventional media. CHROMagar for Staphylococcus aureus was highly specific (97.94%), The specificity increased from 95.24% to 100% when the result was evaluated in conjunction with Mast latex Kit. In the present study, one of the biggest advantage observed was the shortest Incubation period of 24hrs with CHROMagar yields a highest recovery rate of 95% over the conventional media which required 48hrs of Incubation as per the standard method for food analysis.

Published Date: 2024-07-19; Received Date: 2024-06-18