Biochemistry & Pharmacology: Open Access
Open Access

Abstract

Assessment of the Breast Milk Triglyceride, Protein and Its Influencing Factors in Tehran, Iran

Hoda Boushehri, Shahnaz Khaghani, Hossein Mirmiranpour, Sedigheh Shams and Mohammad Zangooei

Introduction: The composition of human milk is the biologic norm for infant nutrition. Human milk also contains many hundreds to thousands of distinct bioactive molecules that protect against infection and inflammation and contribute to immune maturation, organ development, and healthy microbial colonization. Methods: 433 and 216 mothers were selected from Tehran southern and northern health care centers. The mother’s diet was measured by a 24 hour diet history questionnaire for the past 3 days. All breast milk samples were frozen in plastic containers and stored immediately at –20°C until analysis. The samples were centrifuged at 3000 g for 15 minutes and the superficial fat layer was separated. Breast milk protein was measured by the Lowry method. Breast milk Triglyceride was measured by the triglyceride colorimetric assay kit. Results: The breast milk triglyceride and protein by mother's age by age, mother’s occupation and mother’s diet were mentioned in the tables. Discussion: These findings suggest that milk composition is more sensitive to maternal factors such as age, weight, height, occupation, education and mother’s diet. The assessment between mother’s diet carbohydrate and protein between two groups showed a significant difference, statistically (P = 0.001 and P = 0.000) and also between the breast milk protein between two groups showed a significant difference, statistically (P = 0.028). The assessment between the mother’s diet fat in two groups was not significant (P = 0.069) and also the assessment of the breast milk triglyceride between the two groups did not show a significant difference (P = 0.258). Conclusion: There was a significant difference between the mother’s diet in the two groups and help us to change breast feeding malnourished mother’s diet and convert it into a normal diet.