Journal of Chromatography & Separation Techniques
Open Access

Abstract

Direct Determination of Four ACE-Inhibitors Lisinopril, Enalapril, Captopril and Fosinopril in Pharmaceuticals and Serum by HPLC

Sultana N, Naveed S and Arayne MS

The high performance liquid chromatography (HPLC)-UV method for the simultaneous determination of lisinopril, enalapril, captopril and fosinopril in human plasma is proposed. Good separation of the analytes was achieved by gradient RP-HPLC with the mobile phase composed as acetonitrile: water (60:40 v/v) adjusted to pH 3.0 by orthophosphoric acid. Lisinopril, enalapril, captopril and fosinopril were eluted from a Purospher STAR RP-and Hypersil ODS column in 1.8, 2.9, 3.2 and 5.4 min respectively. Good linear relationships were observed for all of the analytes (R2 higher than 0.995). Intra and inter-day precision and accuracy results were 98.0 to 102%. Application of the suggested procedure was successfully applied to the determination of theses compounds in active pharmaceutical preparations, dosage formulations and human serum with high percentage of recovery, good accuracy and precision (no interference of excepients) and this method can be applied to routine clinical analysis