Journal of Applied Pharmacy
Open Access

Abstract

Effect of Extraction Solvents on Total Phenol, Flavonoid Content and Free Radical Scavenging Potential of Saraca asoca, an Indian Medicinal Plant

Shahin Taj RA, Sharath J, Bhagya M

Objective: The present study enumerates the assessment of the differences in biological activities among various solvent extracts of the bark of Ashoka. The aim was to estimate the total phenol content and flavonoid content of various solvent extracts of Saraca asoca bark and to establish the free radical scavenging potential of these solvent extracts.

Methods: A total of 7 solvent extracts was prepared by sequential extraction of Saraca asoca bark, which was screened for antioxidant activities by applying 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method, Ferric reducing antioxidant power assay and Total Antioxidant Activity.

Results: The maximum percentage yield of extract was drawn by utilizing methanol as solvent. The results showed that the ethanolic extract contains strikingly high phenol as well as flavonoid content, which also exhibited remarkably high free radical scavenging activity, in all the three in vitro assays performed.

Conclusion: The current work evidences that phenolic and flavonoids play a crucial role in the antioxidant potential of S. asoca. And that the immensity of radical scavenging behavior is also influenced by the solvent used.

Published Date: 2021-04-05; Received Date: 2020-12-11