Journal of Clinical and Cellular Immunology
Open Access

Abstract

In Vitro Protease Inhibition, Modulation of PLA2 Activity and Protein Interaction Studies of Calotropis gigantea

DSVGK Kaladhar, Govinda Rao Duddukuri, Ramesh K, Varahalarao Vadlapudi and Nagendra Sastry Yarla

Plant protection strategies have been proven to be the most promising approach on selection of protease inhibitors. Protease inhibition and PLA2 activity of white and violet varieties of Calotropis gigantea L., has been studied. Comparative protease inhibition studies on white and violet varieties have shown small variation in protease inhibition. In trypsin inhibition, white variety has not shown inhibition but the violet variety has shown the inhibition at 10 μl concentration. In Protease K inhibition, white variety has not shown inhibition but the violet variety has shown the inhibition at 10 μl and 5 μl concentrations respectively. In Chymotrypsin inhibition, both white and violet varieties have not shown any protease inhibition. The plant has also exhibited PLA2 inhibition activity in blood agar containing egg yolk. Protein interaction network profile has shown interactions with circulatory, neural and immune system components that can modulate and simulate the mechanism in the system approach.