Journal of Clinical and Cellular Immunology
Open Access

Abstract

In-vitro Investigation of Fractionated Extracts of Albizia gummifera Seed Against Leishmania donovani Amastigote Stage

Dereje Nigussie, Geremew Tasew, Eyasu Makonnen, Asfaw Debella, Birhanu Hurrisa, Kelbessa Urga and Adugna Wayessa

Natural products represent a rich and promising source of novel, biologically active chemical entities for treating Leishmaniasis. This study investigates the anti-leishmanial activity of n-butanol, aqueous, and chloroform fractions obtained from crude methanolic extract of Albizia gummifera seed in in-vitro (peritoneal mice macrophage) model after one time treatment. The anti-lesihmanial activity was determined from the minimum inhibitory concentration (MIC), infection rate (IR) and multiplication index (MI) against L. donovani. Cyto-toxicity of the fractions was also assessed against Vero cells. In addition to this secondary metabolites were determined by phytochemical screening. The MIC value of n-butanol, aqueous, chloroform and amphotericin B were 11.2 μg/ml, 33.5 μg/ml, >89 μg/ml and 1.32 μg/ml, respectively. N-butanol and aqueous fractions significantly inhibit the growth of intra-cellular L. donovani amastigote (P>0.05) compared to amphotericin B whereas the chloroform fraction did not revealed any significant leishmanicidal effect (P<0.05) with their respective parasitic infection rate, multiplication index and MIC levels. Based on these data, the n-butanol and aqueous fractionates of A. gummifera seeds exhibit considerable in-vitro anti L. donovani activity and may be promising anti-leishmanial herbal remedy candidate that support the traditional use of the plant.