Journal of Clinical and Experimental Ophthalmology
Open Access

Abstract

Erufosine Coated Hydrophilic Intraocular Lenses Attenuate PCO Formation in Vitro

Liegl R, Wertheimer C, Wolf AH, Kampik A and Eibl-Lindner KH

Purpose: To assess the impact of laminin and collagen IV on the proliferation of human lens epithelial cells (LECs) and the performance of two hydrophilic and one hydrophilic IOL with a hydrophobic surface coated with erufosine on their properties to inhibit LEC proliferation and migration in a well-established in vitro anterior chamber model of PCO.

Setting: Research Laboratory for Experimental Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.

Design: Experimental study.

Methods: Three IOLs were selected after pre-evaluation for their properties to inhibit LEC proliferation in vitro. All three IOLs were then coated with erufosine while uncoated IOLs of the same type and lot served as controls. Twelve well cell culture inserts were coated with either laminin or collagen IV and erufosine coated or uncoated IOLs were placed on these inserts into the wells. The wells were inserted into 12 well plates and kept under standard cell culture conditions for 6 days. After removal of the IOLs, the cell culture inserts were analyzed for LEC proliferation and migration.

Conclusions: Collagen IV has a stronger impact on LEC proliferation in vitro than laminin has. All tested erufosine coated IOLs were able to significantly decrease the formation of PCO in an in vitro anterior chamber model.