Journal of Plant Biochemistry & Physiology
Open Access

Abstract

Estimation of Asiaticoside by Using RP-HPLC and FAME Analysis of Medicinally Important Plant Centella asiatica

Arpita Roy, Koyel Kundu, Gaurav Saxena and Navneeta Bharadvaja

Due to the presence of several bioactive compounds, Centellaasiatica (L.) is used in traditional medicine for the treatment of various ailments. This usage of plant and its products created a huge demand which required an alternative method of its commercial production so that the loss of natural plant and its genetic pool can be prevented. A different investigation using biotechnological methods provided a wealth of information to enhance the biomass as well as different bioactive compounds. In the present study, we focused and reported the effect of different nitrogen sources on shoot proliferation of Centellaasiatica. Effect of malt extract as plant elicitor on asiaticoside production as well as fatty acid methyl ester (FAME) profile was also worked out. Four different nitrogen sources i.e. NH₄NO₃ (1.65 g/l), KNO₃ (0.8 g/l), NaNO₃ (1.65 g/l), Ca(NO₃)₂ (0.825 g/l) on five different accessions of Centellaasiatica were tested and accession number 347492 (14.66 ± 2.4) provided maximum shoot proliferation with ammonium nitrate. RP-HPLC analysis of accession number 347492 revealed that malt extract as plant extract enhanced 6 times asiaticoside in comparison to standard conditions. GC-MS analysis of five different accessions of Centellaasiatica concluded that this plant is rich in the content of Pentadecanoic acid, 9,12 Octadecadienoic acid (Linoleic acid) and 9,12,15 Octadecatrienoic acid (Linolenic acid). Most potential fatty acid methyl esters containing plant accession number is 281374.