Journal of Probiotics & Health
Open Access

Abstract

Evaluation of Potential Anticancer Activities of Prebiotics, Probiotics and Synbiotics: In Vitro Study

Ahmed Fazary*, Eman Ismael, Hayam Fathy, Mohammad Alfaifi, Serag Eldin Elbehairi and Doaa Badr

Three independent experiments have been performed for three human cancer cell lines: Adenocarcinomic Human Alveolar Basal Epithelial Cells, (A549), Hepatoblastoma Cell Line (HepG2) and Cancer Coli-2 (CaCo-2), where test samples were divided into six groups: Grape seed extract and licorice root extract as prebiotics, Lactobacillus acidophilus bacteria supernatant and cell sonicate as probiotic and combination of prebiotic and probiotic. Cell viability variations were evaluated, while further anticancer activity was assessed via flow cytometry for cell cycle arrest profile and caspase 3 expressions was monitored. Obtained results showed that IC₅₀ was reduced by the combined administration of pre and probiotics more than indicted by probiotics individually. More prominently observed in HepG2 cell line compared to CaCo-2 and A549 cell lines respectively. Data revealed that the variation of the apoptotic profile was tested extract formulae dependent. Cells treatment induced a significantly elevated early apoptotic than in the case of control cells, while grape mix showed a significantly elevated late apoptotic than in the case of cells treated with grape extract and licorice mix. Also, there was an insignificant necrotic cell in the case of cell treatment with grape, grape mix and licorice mix as well. At the same time, it was noticed that the amount of active caspase-3 present in each culture sample was increased in cells treated with test samples more than in control cells by an obvious percentage.

Published Date: 2024-09-20; Received Date: 2024-08-20