Journal of Clinical and Experimental Ophthalmology
Open Access

Abstract

Exposure to Subthreshold Dose of UVR-B Does Not Induce Apoptosis in the Rat Lens In Vivo within First Twenty Four Hours

Konstantin Galichanin*, Lovisa Falkman, Zhaohua Yu

Purpose: The aim of this study is to investigate the time evolution of active caspase 3 within first 24 hours in the rat lens after in vivo exposure to subthreshold dose of UVR-B. Methods: Twenty-six-week-old female albino Sprague-Dawley rats were exposed to subthreshold dose (1 kJ/m2 ) of UVR-B unilaterally and sacrificed at 1, 8, 16 and 24 hours after exposure. Lenses were enucleated and active caspase 3 was detected by western blot. The time evolution of active caspase 3 was then plotted as a function of relative mean difference in active caspase 3 between exposed and nonexposed lenses. Results: There is expression of active caspase 3 in both exposed and nonexposed lenses but there is no difference in relative mean difference in active caspase 3 between exposed and nonexposed lenses in all four postexposure groups. Conclusions: Exposure to subthreshold dose of UVR-B does not induce apoptosis in the rat lens in vivo within first 24 hours. Apoptosis occurs in the rat lens in vivo independently from the exposure to subthreshold dose of UVR-B.

Published Date: 2025-01-29; Received Date: 2024-11-01