Journal of Chromatography & Separation Techniques
Open Access

Abstract

Extraction and Purification of Anthocyanins from the Fruit Residues of Vaccinium uliginosum Linn

Zhang Hua, Dong Yuesheng, Xu Ge, Li Menglu, Du Liya, An LiJia and Xiu Zhilong

An aqueous two-phase system was developed to extract anthocyanins present in the fruit residue during juice production from the fruit of Vaccinium uliginosum Linn. A maximum partition coefficient of 10.67 and a recovery of 96.09% for anthocyanins could be obtained using an extraction system consisted of 30% (w/w) ethanol and 19% ammonium sulfate. Compared with the traditional extraction using acidified ethanol, the novel aqueous two-phase extraction could not only yield a much higher concentration of anthocyanins, save more ethanol, energy, and time, but also decrease impurities in extract, e.g. proteins and sugars by 58% and 66%, respectively. AB-8 macroporous resin was applied to the purification of anthocyanins. A novel and simple separation technique for anthocyanins was developed by integrating aqueous two-phase extraction and macroporous resin column chromatography. This new technology might be a suitable for other bioactive natural products on industrial scale.