Virology & Mycology
Open Access

Abstract

Further Studies on the Antiviral Agent Isolated from Host Plants, Pretreated with Boerhaavia diffusa Glycoprotein

L.P. Awasthi, S.P. Singh, H.N. Verma and S. Kluge

Induced antiviral agent(s), following treatment with Boerhaavia diffusa glycoprotein in several host plants, was purified by fractionation with organic solvents followed by precipitation with ammonium sulphate, dialysis, ultracentrifugation and filtration through Amberlite, Polyamide, Silica gel, DEAE cellulose and Sephadex gel columns. Biologically active material was eluted in two different fractions. Purified Antiviral Agent(s) (AVA) was active selectively against anisometric and isometric viruses. It was hydrolyzed by Trypsin and Pronase but not affected by RNAs or DNAs. It showed protein nature. Molecular weight of AVA fractions was 8000-10,000 and 32,000-35,000 daltons.