Journal of Proteomics & Bioinformatics
Open Access

Abstract

In Silico Structural and Functional Prediction of Phaseolus vulgaris Hypothetical Protein PHA VU_004G136400g

Zohaib Bashir, Muhammad Rizwan, Kanwal Mushtaq, Anum Munir and Ishtiaq Ali

Hypothetical proteins (HPs) are the proteins whose occurrence has been predicted, yet in vivo function has not been manufactured up. Illustrating the structural and functional confidential visions of these HPs might likewise prompt a superior understanding of the protein-protein relations or networks in diverse varieties of life. Common bean (Phaseolus vulgaris) is the most important food legume for direct human consumption in the world, most important grain legume for human consumption and has a role in sustainable agriculture helping to its ability to fix atmospheric nitrogen. However, in spite of the importance of this crop species, its genetics have been poorly characterized. In the present study, the hypothetical protein of Phaseolus vulgaris (Common bean) was chosen for analysis, and modeling by distinctive Bioinformatics apparatuses and databases. As indicated by primary and secondary structure analysis, XP_007152511.1 is a stable hydrophobic protein containing a noteworthy extent of α-helices; Homology modeling was utilized SWISS-MODEL server where the templates identity with XP_007152511.1 protein was less which demonstrated novelty of our protein. The Ab initial strategy was conducted to produce its 3D structure. A few evaluations of quality assessment and validation parameters determined the generated protein model as stable with genuinely great quality. Functional analysis was completed by ProtFun 2.2, and KEGG (KAAS), recommended that the hypothetical protein is a translation factor with nuclear domain. The protein was observed to be energetic for translation process, involved in trans-membrane barriers, signaling and cellular processes, and protein binding. It is suggested that further test approval would help to anticipate the structures and functions of other uncharacterized proteins of different plants and living being.