Pharmaceutical Analytical Chemistry: Open Access
Open Access

Abstract

Investigation of Ginsenoside Rb1 from Acanthopanax koreanum by Eastern Blotting and ELISA Analyses

Nguyen Huu Tung, Takuhiro Uto, Susumu Isoda and Yukihiro Shoyama

Ginsenosides such as ginsenoside Rb1 are the principal components in Panax spp. and have been found in some other Araliaceae plants. In our survey of ginsenoside from Araliaceous species based on eastern blotting and ELISA methods using antiginsenoside Rb1 monoclonal antibody, ginsenoside Rb1, one of the principal ginseng saponins, was investigated with concentrations of 0.000016, 0.000014, and 0.000039% dry weight in the leaves, stem, and roots of the well-known medicinal plant Acanthopanax koreanum respectively. The obtained results further support potential and promising application of MAb including eastern blotting and ELISA for surveying ginsenoside sources including ginsenoside Rb1.