Journal of Chemical Engineering & Process Technology
Open Access

Abstract

Isolation, Purification and Characterization of β-Glucosidase from Rauvolfia serpentina.

Om Prakash Verma, Abha Singh, Nirmala Singh and Onkar Chaudhary

β-Glucosidase (EC 3.2.1.21) was extracted from different parts of Rauvolfia serpentina and was purified using ammonium sulphate fractional precipitation and Sephadex G-25 chromatography. The molecular weight of different bands in Rauvolfia serpentina was found in the range of 19.4KDa to 92.257KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60ºC while the thermal stability of enzyme β-glucosidase was found to be 30ºC. The activation with FeSO4 is due to Fe++ ions because FeCl3 do not activate the enzyme.