ISSN: 2736-6588
+44 1223 790975
Ajaikumar Sukumaran, Thushara Thomas, Deepa K Vijayan, Jofy K Paul
The C-reactive protein is a pentameric, annular, acute phase protein encoded by the chromosome-1 in humans. This hepatogenic protein shoots up in the blood in response to most of the bacterial and viral infections. Hence C-reactive protein is considered as the diagnostic as well as prognostic marker in the treatment of infections. Recently the high sensitive C-reactive protein in the blood is used as a trustworthy marker for the diagnosis of cardiovascular diseases. We have standardized an immunoassay reagent for quantitative C-reactive protein detection based on latex based immunoturbidimetry, which offers the detection of C-reactive protein in a wide range of 0.2-320 mg/L with an accuracy of >99%. The developed immunoassay reagent facilitates the analytical sensitivity of 0.2 mg/L and analytical linearity of 320 mg/L. The standardized reagent has been internally validated with a total number of 100 serum samples and the performance studies have been conducted with biochemistry quantitative autoanalyzer Toshiba-25 FR with reference to the immunonephelometry analyzer, the Siemens BN-Prospec.
Published Date: 2020-09-03; Received Date: 2020-08-12