Gynecology & Obstetrics
Open Access

Abstract

Macrophage Colony-Stimulating Factor Modulates Matrix Metalloproteinase 2 and 9 Expression in Endometrial Epithelial Cells

Haifaa A Mansouri, Nameer B Kirma, Peter A Binkly, Naveen K Krishnegowda and Rajeshwar R Tekmal

Objective: To investigate m-Colony Stimulating Factor (CSF-1) effect on the expression of Matrix Metalloproteinases (MMP) 2 &-9 by primary endometrial epithelial cells (EECs) and Endometrial Stromal Cells (ESCs).
Design: In vitro study. Main Outcome measure: the expression of MMP 2 &-9 by EECs and ESCs.
Results: MMP-2 expression and activity of primary epithelial cells significantly decreased in CSF-1 treated EECs compared to control vehicle (P Value<0.02). A non-significant decrease in MMP-9 RNA expression and activity was observed in EECs due to CSF-1 treatment. MMP-9 activity was significantly higher in ESCs than in EECs (P Value<0.004). 26122013.
Conclusion: This study suggests that CSF-1 modulates MMP-2 expression and activity in EECs and may regulate MMP-9 via transcriptional and post-transcriptional mechanisms. Future studies are needed to further address the complex regulation of MMP expression in endometriotic lesions.