Chemotherapy: Open Access
Open Access

Abstract

MiR-188-5p and MiR-141-3p Promote Bladder Cancer Synergistically via Targeting PTEN to Activate AKT/c-MYC Signal Pathway

Xianxu Yang, Zongze Li*

MicroRNAs play a key role in the progress of Bladder Cancer (BC), which may lead to poor prognosis. A single MicroRNA can be used as an independent factor to regulate the progress of BC, while two MicroRNAs can have a synergistic regulatory effect on BC progress. It has been confirmed in our previous studies that miR-188-5p and miR-141-3p demonstrated high expressions in BC tissues and cells, which can promote the progress of BC and affect patient’s prognosis. As a follow-up research, this study has made further exploration in many aspects. We predicted and confirmed that miR-188-5p and miR-141-3p had a common target gene PTEN which had low expression in BC tissues and cells. Down regulation of PTEN can promote the progress of BC, and significantly reverse the inhibitory effect of down-regulated miR-188-5p and miR-141-3p on BC progress. PTEN is a cancer suppressor gene. Experiments further verified that pAKT and c-MYC were downstream effector proteins of PTEN, and their expressions increased with the decrease of PTEN expression. Experiments manifested that down-regulating miR-188-5p and miR-141-3p could significantly inhibit the volume and weight of subcutaneous tumors in mice, and half dose co-transfection of the two miRs made the tumor smaller and lighter. Therefore, it was concluded that miR-188-5p and miR-141-3p promoted the progress of BC synergistically by inhibiting PTEN to activate AKT/c-MYC pathway.

Published Date: 2025-01-24; Received Date: 2023-10-27