Journal of Chromatography & Separation Techniques
Open Access

Abstract

Modification of a Targeted Metabolomics Method Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry to Measure Nine Purines in Commercial Dog Foods

Chie Saito Ishii*, Karen C Scott, Carolina Abrahan, Joy G. Cagmat, Taylor A. Harmon, Yann Queau, Vincent Biourge, Timothy J. Garrett and Richard C Hill

Urate is the third most common component of canine uroliths. Dogs with a genetic mutation or hepatic dysfunction are prone to forming urate uroliths because they excrete more poorly soluble urate as the end product of purine metabolism. Dietary purine restriction has been reported to reduce urinary urate concentrations in dogs, but the amount and types of purine in commercial diets have not been well documented. A previous targeted metabolomics method was modified to measure eleven purine metabolites in commercial dog foods. The method utilized hydrophilic interaction liquid chromatography-high-resolution tandem mass spectrometry with stable isotope-labeled internal standards. The within-day and between-day coefficient of variation of each purine metabolite concentration in quality control dog food samples averaged 6% (range: 4%-13%) and 15% (range: 11%-19%), respectively. Recoveries were within 100% ± 20% for adenine, guanine, xanthine, adenosine, inosine, guanosine, and adenosine monophosphate at added purine concentrations similar to those in a low and a high purine food chosen to assess quality control.

Published Date: 2023-12-28; Received Date: 2023-11-18