Journal of Cancer Research and Immuno-Oncology
Open Access

Abstract

Non-proliferating Effect of Syzygium cumini and Spondias pinnata Against Colorectal Carcinoma

Yalachigere Kempaiah Suneetha*, Hongasandra Lakshmipathy Neha, S. Anand

Background: In today’s world the use of medicine, especially of plant origin, is acquiring a lot of significance in restorative applications because of the relatively low harmfulness when contrasted with designed pharmaceuticals.

Methods: Methanolic extracts of Spondias pinnata, and Syzygium cumini were evaluated for antioxidant properties using DPPH and ABTS radical scavenging assays. MTT assay for the extract was carried out against the HCT116 cell line. HCT1116 cells treated with 40 and 80 μg/mL of S. cumini were studied using Annexin V FITC flow cytometry. PCR analysis of the caspase3 gene in HCT116 cells treated with 40 and 80 μg/mL of S. cumini extract was done to confirm apoptosis. A hemolysis assay was performed to determine the toxicity effect of the plant extracts on human RBCs.

Results: IC₅₀ of S. cumini and S. pinnata were found to be 23.72 and 56.51 μg/mL; 47.64 ug/mL and 19.7 μg/mL for DPPH and ABTS radical scavenging activity. MTT assay showed an IC₅₀ value of 59.43 and 137 μg/mL for S. cumini and S. pinnata respectively. Flow cytometry study indicated that 17.2 and 4%, and, 20.49% at a concentration of 40, 80 μg/mL have attained early apoptosis while the rest have attended late apoptosis and necrosis. PCR analysis of caspase3 indicates that S. cumini has demonstrated superior anti-cancer activity against HCT116 cells.

Conclusion: Downstream studies with flow cytometry on apoptosis and PCR analysis of the caspase3 gene suggest that S. cumini has comparably shown better anti-cancer activity against HCT116 cells proving the potential as a therapeutic agent.

Published Date: 2025-01-09; Received Date: 2023-02-26