Journal of Chromatography & Separation Techniques
Open Access

Abstract

Optimizing UHPLC Fittings and Connections: A Case Study

Eduard Rogatsky, Shaynah B, Cai M and Daniel TS

We experienced persistent problems in achieving robust connections in UHPLC applications utilizing sub-3 micron particle size reversed phase chromatographic columns. Our application involved column testing, which involves frequent disconnection and re-connection of different columns into/from the flow path ofthe UHPLC system. In this paper we report our findings and recommendations.