Anatomy & Physiology: Current Research
Open Access

Abstract

Particle dose dependent neurotoxic effect of silver nanoparticles in fetuses of Swiss Albino mice

Pani J P,Singh R,Shamal S N, Mishra S P, Gaur P, Gupta R

Nanoparticles exhibits idiosyncratic properties because of that it shows wide range of applications in industries and many biomedical sectors ultimately leads to nanopolution. AgNps effects on embryo-fetus neuro-development are still under research level. To evaluate dose dependant neurotoxic effect of silver nanoparticles in pregnant Swiss albino mice and their fetuses. The present study was undertaken to determine the potential effects of AgNps of different dose on brain of pregnant dams and fetus after repeated maternal exposure on gestational days (GD) 5–17 in mice. AgNps colloidal solution, were administered to pregnant mice by repeated oral gavages at concentrations of 0.5, 1, 5, 10, 15 & 20 mg/kg/day respectively. Brain removals were done on all dams and fetuses on GD 18. Samples were exposed to H&E and Golgi staining. The dams and fetuses were evaluated for signs of neurotoxic effects. Maternal and Fetal nervous tissue showed significant histomorphological alterations in the form of Gliosis, vacuolization and honey comb shape deformity of neural cells in H&E 40x view. Whereas Golgi stain of fetus brain in same magnification showed damaged dendritic arborization and weak multipolar neuron. The intensity of such sign and symptoms were observed higher in 5, 10, 15 and 20 mg/kg/day dose group and lower in 0.5 and 1mg/kg/day dose group. The results show that a repeated oral dose of AgNps during pregnancy caused oxidative stress and neuro toxic effects in maternal and fetal brain tissue which is dose dependent

Published Date: 2020-06-30; Received Date: 2020-05-30