Journal of Chromatography & Separation Techniques
Open Access

Abstract

Phytochemical Characterization of Active Constituents from Extracts of Ixora Javanica D.C Flowers

Sunitha Dontha, Hemalatha Kamurthy and Bhagavan Raju Mantripragada

The investigation of chemical compounds from natural products is fundamentally important for the development of new drugs, especially in view of the vast worldwide flora. The present work is extraction, isolation and characterization of active constituents from Ixora javanica D.C flowers. Freshly collected flowers were shade dried, powdered and subjected to continuous soxhilation with various solvents like petroleum ether (60-80°), ethyl acetate and ethanol (70%) of increasing polarity. All the three obtained extracts were concentrated and after preliminary phytochemical investigation, column chromatographed using silica gel. Three triterpenoidal compounds (Maslinic acid, Ursolic acid and Lupeol), one flavonoidal compound (Formononetin) and two flavonoidal glycoside compounds (Quercetin-3-rutinoside, Quercetin-3-glucoside) have been isolated and further these isolated structures were established by spectral analysis (IR, 1H NMR spectra, 13C NMR and Mass) and direct comparison with authentic samples. This is the first report of occurrence of these compounds from Ixora javanica D.C flowers.