Journal of Chromatography & Separation Techniques
Open Access

Abstract

Purification of the Mule (Equus mulus) IgG by Protein A - Affinity Chromatography

Justiz-Vaillant AA, McFarlane NA, Mohammed W and Sehlule Vuma

A mule is the off-spring (F1 hybrid) of two equine species: a male donkey and a female horse. Little is known about the immune system of the mule. In this investigation we isolated and characterized molecularly the mule IgG that was purified by Staphylococcal protein A (SpA) affinity chromatography, and then the purified IgG was further study by SDS-PAGE and enzyme-linked immunosorbent assay that concluded that the intact mule IgG has a molecular weight of 150 kDa.