Journal of Tumor Research
Open Access
ISSN: 2684-1258
ISSN: 2684-1258
KVL Narasingha Rao, Omkar Shirke, Vivek Ghose, Rahul R Patil, Yogananda S Markandeya, Periyasamy Govindaraj, Bhupesh Mehta* and Manish Beniwal*
Craniopharyngioma although benign, is one of the most difficult tumors to resect and manage. The fluid inside the tumors is known to accumulate even after aspiration leading to tumor recurrence. Spontaneous rupture of the tumor leads to leakage of the cyst fluid to the surrounding tissue and is known to cause aseptic meningitis. This study aimed to identify toxic proteins in the cyst fluid which could be responsible for altering the normal physiology of the surrounding brain tissue. We first characterized the proteomic content of the cyst fluid collected from 12 patients by tandem mass spectrometry which revealed 91 common proteins. These proteins were then subjected to bioinformatics analysis to identify the biological processes, cell components and molecular functions of the proteins and were further compared with the proteins involved in neurodegenerative diseases to narrow our search for toxic proteins. 46 out of the 91 proteins were taken for functional enrichment on Cytoscape. Functional enrichment then revealed a total of 21 toxic proteins in the fluid which could play a role in the regulation of cell death or are involved in cell killing. The interaction of a repertoire of proteins involved in metastasis, inflammation, extracellular matrix reorganization and cell death as evidenced by the protein-protein interaction network suggests the interplay of the proteins in the cyst fluid, which could directly or indirectly be cytotoxic. Data are available via proteome exchange with identifier PXD051226.
Published Date: 2024-10-04; Received Date: 2024-09-02