Advanced Techniques in Biology & Medicine
Open Access

Abstract

Sandwich ELISA for Circulating Myeloperoxidase- and Neutrophil Elastase-DNA Complexes Released from Neutrophil Extracellular Traps

Hideki Kano, Muhammad Aminul Huq, Masanobu Tsuda, Hiroshi Noguchi and Naoshi Takeyama

Neutrophil extracellular traps (NETs) are DNA scaffolds released by activated neutrophils that contain enzymes from neutrophil granules, such as myeloperoxidase (MPO), neutrophil elastase (NE) and cathepsin-G. NETs are produced by neutrophils in response to various stimuli, including invasion of pathogenic microorganisms. Here we describe a new ELISA method for quantifying the circulating levels of MPO- and NE-associated DNA in human plasma.