Journal of Chromatography & Separation Techniques
Open Access

Abstract

Simultaneous Estimation of Metformin Hydrochloride and Repaglinide in Pharmaceutical Formulation by HPTLC-Densitometry Method

Keyur B. Ahir, Emanual Michael Patelia and Arpit Shah

A simple, precise, rapid, selective, and economic reversed phase high-performance liquid chromatography (HPTLC) method has been established for simultaneous analysis of Metformin Hydrochloride and Repaglinide. HPTLC method was developed using on precoated silica gel G60 F254 plates as stationary phase, using methanol:ammonium sulphate (0.25%) (pH-5.7) (2.5:7.5, v/v) as mobile phase. The plates were scanned at approximately 243 and 236 nm for HPLC and HPTLC both respectively. In HPTLC method both the drugs were resolved using proposed mobile phase and Rf value was found to be 0.34 for MET and Rf 0.60 for REPA. The method was found to linear in the range 500-2500 ng/band for MET, and 100-500 ng/band based for REPA respectively. This HPTLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis of combined dosage form.