Cell & Developmental Biology
Open Access

Abstract

The Comparison of Single-Cell RNA Sequencing Platforms Based Dropletsxianhong

Yulong Zhong, Linyan Wang and Xianhong Yu*

Single-cell sequencing enables to reveal cellular heterogeneity and discover new cellular subpopulations. In terms of the strategy of single-cell sequencing, the main methods are based with combinatorial index, microwell and microfluidic. Due to the simplicity, methods based droplets are widely used for single-cell sequencing for multi-omics. Therefore, in order to facilitate researchers to choose a suitable platform to meet their application scenarios, we compared several commercial platforms: The Chromium X platform of 10x Genomics, the MobiNova-100 platform of MobiDrop, the SeekOne platform of SeekGene, and the C4 platform of BGI. Based the comprehensive assessment of the data analysis, the Chromium X platform shows an excellent performance, closely followed by MobiNova-100 platform.

Published Date: 2024-10-11; Received Date: 2024-09-11