Applied Microbiology: Open Access
Open Access

Abstract

The Hypoglycaemic Effect of the Components from Pleurotus eryngii was evaluated on model In vitro and In vivo

Jinmei Lin, Jingna Liu, Jiaofen Lin, Jiafu Huang, Zhichao Lin, Bo Leng, Yu Xue, Yi Su, Liyun Yao, Xiumin Li, Yuanhong Zhuang and Yutian Pan

Four structural substances were extracted from P. eryngii, including PEE-1, PEE-2, PEE-3 and PEE-4. The chemical analysis was conducted to measure the contents of polysaccharide and protein in PEE-1, results found that they were 10.78% and 26.66% respectively. PEE-2, PEE-3 and PEE-4 were respectively identified as β-glucan, chitosan and D-glucosamine hydrochloride by FT-IR, GC, HPLC and HPLC-MS. The results showed that PEE-1, PEE-2 and PEE-3 could inhibit the activity of α-glycosidase from the rabbit isolated small intestine, while the PEE-4 had no inhibitory effect. At the same concentration, the PEE-1 showed more significantly inhibitory effects on α- glycosidase activity than Glucobay (P<0.05). The hypoglycemic effect of PEE-1 had significant differences in the blood glucose between each group and model control group (P<0.05). In glucose tolerance test, the other groups had significant differences compared with the model control group (P<0.05). In addition, high dose of PEE-1 normal group had no significant effect on the fasting blood glucose level with normal control group (P>0.05). Results showed that PEE-1 had the hypoglycaemic function in hyperglycaemia animals, and it could be applied in the research and production of hypoglycaemic health food.