Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
Abu-Farich B
Israel
Research Article
Hypericum triquetrifolium Extracts Modulate IL-6, IL-10 and TNF-α
Protein and mRNA Expression in LPS-Activated Human Peripheral Blood
Mononuclear Cells and THP-1-Derived Macrophages
Author(s): Saad B, Embaslat WH, Abu-Farich B, Mahajna S, Azab M, Daragmeh J, Khasib S and Zaid HSaad B, Embaslat WH, Abu-Farich B, Mahajna S, Azab M, Daragmeh J, Khasib S and Zaid H
The aim of the present in vitro study was carried out to evaluate the anti-inflammatory activity of Hypericum triquetrifolium extracts (HT-extract) on lipopolyssacharide -stimulated human monocytic (THP-1) cells and human peripheral blood mononuclear cells (PBMNCs). The expression and production of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6), as well as the anti-inflammatory cytokine interleukin 10 (IL-10) were evaluated by assessing the levels of proteins and mRNA’s of TNF-α, IL-6 and IL-10 in both cell types. Cells were exposed to 5 μg lipopolyssacharide (LPS) /ml in the absence and presence of increasing concentrations of 50% ethanol extracts from the aerial parts of Hypericum triquetrifolium. The anti-inflammatory efficacy experiments were performed with HT-extract concentrations up to 250 μg/ml that had no cyto.. View More»
DOI:
10.4172/2167-0412.S3-004