ISSN: 2329-9029
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Aida Abraha
Tanzania
Research Article
Medicago sativa L. β-Amylase Core Promoter has Motifs in Common with Arabidopsis Key Starch Degradation Genes
Author(s): Brett Fugate, Denise Medrano, Joyce Ache Gana, Aida Abraha and Suzanne M Cunningham
Brett Fugate, Denise Medrano, Joyce Ache Gana, Aida Abraha and Suzanne M Cunningham
β-amylase is an enzyme involved in the degradation of transitory starch in photosynthetic tissues of plants and
is expressed in high levels in the roots of Medicago sativa L. How the β -amylase gene MsBAM1 is regulated in
the roots is not clear. The aims of this study were to: isolate the core promoter of MsBAM1, decipher motifs in silico
that are associated with the elevated root specific response, and to find common motifs among core promoters
of important starch degradation genes in Medicago sativa MsBAM1 and Arabidopsis (AtGWD, AtPWD, AtMEX1,
AtBAM 1 & 3, and AtISA3). Primers were designed from homologous β-amylase genes and used in PCR with
M. sativa gDNA. Gel purified PCR band(s) were sequenced, verified and annotated. The starch genes and equal
numbers of Arabidopsis non-starch.. View More»
DOI:
10.4172/2329-9029.1000124