Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Alan J Tackett
Tanzania
Research Article
CD109 Overexpression in Pancreatic Cancer Identified by Cell-Surface
Glycoprotein Capture
Author(s): Randy S Haun, Chun-Yang Fan, Samuel G Mackintosh, Hong Zhao and Alan J TackettRandy S Haun, Chun-Yang Fan, Samuel G Mackintosh, Hong Zhao and Alan J Tackett
Background: The development of novel targeted cancer therapies and/or diagnostic tools is dependent upon an understanding of the differential expression of molecular targets between normal tissues and tumors. Many of these potential targets are cell-surface receptors; however, our knowledge of the cell-surface proteins upregulated in pancreatic tumors is limited, thus impeding the development of targeted therapies for pancreatic cancer. To develop new diagnostic and therapeutic tools to specifically target pancreatic tumors, we sought to identify cell-surface proteins that may serve as potential tumor-specfic targets.
Methods: Membrane glycoproteins on the pancreatic cancer cell lines BxPC-3 were labeled with the bifunctional linker biocytin hydrazide. Following proteolytic digestion, biotinylated glycopeptides were captured with streptavidin-coupled beads then released by PNG.. View More»
DOI:
10.4172/jpb.S10-003