ISSN: 2376-0354
+44-77-2385-9429
Alexander Schaller
Tanzania
Research Article
Crossing the Finish Line: How to Develop Diagnostic DNA Tests as Breeding Tools after QTL Discovery
Author(s): Vanderzande S, Piaskowski JL, Luo F, Edge-Garza DA, Klipfel J, Schaller A, Martin S and Peace C*
Vanderzande S, Piaskowski JL, Luo F, Edge-Garza DA, Klipfel J, Schaller A, Martin S and Peace C*
DNA-informed breeding, the integration of DNA-based genetic information into plant breeding programs, can enhance efficiency, accuracy, creativity, and pace of new cultivar development. Most genetic knowledge of key traits for plant breeding has been obtained through QTL analyses. Despite an explosion in QTL discoveries for horticultural crops, very few of those discoveries have been translated into tools for horticultural crop breeding. An example of such tools with direct application in crop genetic improvement are trait-predictive DNA tests. The translation of a promising QTL to a trait-predictive “DNA test” has five steps: (1) choose target QTL; (2) design assay to target locus; (3) assay individuals; (4) trace inheritance; and (5) disseminate DNA test details. Key information to convey to end users about a DNA test are the crop and trait(s) addressed, targeted trait l.. View More»
DOI:
10.4172/2376-0354.1000228