Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Alexander V Stoyanov
Tanzania
Research Article
Simultaneous Monitoring of Multiple Transitions in Mass Spectrometric Analysis Improves Limit of Detection for Low Abundance Substances in
Complex Biological Samples
Author(s): Dmitriy Shin, Eduard Rogatsky and Alexander V Stoyanov
Dmitriy Shin, Eduard Rogatsky and Alexander V Stoyanov
In mass-spectrometry, simultaneous monitoring of several transitions enables increased assay specificity, but also can be used as a tool to improve the lower limit of quantification. In this report, we propose a new approach to low abundant analyte detection in complex samples. The method consists of collecting two and potentially more, different mass spectrometry transitions with further analysis of a product function. For example, C-peptide detection was evaluated with two transitions monitored (1007.7-147.2 and 1007.7-927.8). Further extension of the method is theoretically analyzed and its potential limitations are discussed.
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DOI:
10.4172/2157-7064.1000206