Journal of Proteomics & Bioinformatics
Open Access

Anthony J Janckila

Publications

• Research Article
  Proteomic Analysis of Rat Prefrontal Cortex after Chronic Lithium Treatment
  Author(s): Jaganathan Lakshmanan, Ratnam S Seelan, Muthusamy Thangavel, Robert E Vadnal, Anthony J Janckila, Manuel F Casanova, Latha K Parthasarathy and Ranga N Parthasarathy Jaganathan Lakshmanan, Ratnam S Seelan, Muthusamy Thangavel, Robert E Vadnal, Anthony J Janckila, Manuel F Casanova, Latha K Parthasarathy and Ranga N Parthasarathy
  
  Although lithium is widely used to treat Bipolar disorder (BD), its therapeutic role in BD is unclear. To gain insights into its mechanism of action we have used proteomic analysis to identify differentially expressed proteins in rat Prefrontal cortex (PFC), a region specifically affected in BD, after six weeks of lithium treatment. Proteins from control and lithium treated rat PFCs were separated by 2 Dimensional - Differential In-Gel Electrophoresis (2D-DIGE) and identified by mass spectrometry. Of the 2198 protein spots resolved, the abundance of 19 proteins was found to be significantly altered in the lithium treated group (with the levels of 5 proteins increasing and those of 14 decreasing). The levels of two protein spots exhibiting significant alteration after chronic lithium exposure were verified by Western blot analysis of rat PFC extracts. The 19 identified proteins represe.. View More»
  DOI: 10.4172/jpb.1000226

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