ISSN: 2167-0250
+44 1300 500008
Antonino Pipitò
Italy
Research Article
Oxidative Stress Evaluation in Sperm Samples from Fertile and Infertile Men
Author(s): Andor Crippa, Maria C Magli, Anna P Ferraretti, Antonino Pipitò, Edoardo Pescatori and Luca Gianaroli
Andor Crippa, Maria C Magli, Anna P Ferraretti, Antonino Pipitò, Edoardo Pescatori and Luca Gianaroli
Background: Transition metal ions, such as iron, can make electron donations to oxygen forming superoxide or hydrogen peroxide, which is further reduced to an extremely reactive hydroxyl radical that induces oxidative stress. The purpose of the present study was to design a system that could easily detect and reliably measure the ferrous oxidation associated to oxygen radical reactions in the sperm samples. Methods: A total of 64 sperm samples from 11 men who had normal semen parameters and proven fertility and 53 male partners of couple experiencing primary infertility, were included in the study. The semen samples from oligoasthenoteratozoospermic patients was divided on the basis of spermatic parameters into moderate, when the sperm concentration was ≥5 × 106/ml and in severe when the concentration was <5 × 106/ml. The evaluation of the ferrous oxidation was perfo.. View More»
DOI:
10.4172/2167-0250.1000S1-002