Journal of Chromatography & Separation Techniques
Open Access

Awet GT

Publications

• Research Article
  A Simple and Sensitive Method for Quantitative Measurement of Methylmalonic Acid by Turbulent Flow Chromatography and Tandem Mass Spectrometry
  Author(s): Awet GT, Ryan CS, Lakshmi VR and Dean C Carlow Awet GT, Ryan CS, Lakshmi VR and Dean C Carlow
  
  A simple and sensitive method for the detection of methylmalonic acid in serum without derivatization has been developed. This method implements protein precipitation using methanol followed by additional sample clean up by turbulent flow liquid chromatography (TFLC). The sample was directly injected into the turbulent flow liquid chromatography tandem mass spectrometrysystem (TFLC-MS/MS) for online extraction followed by HPLC separation. The eluent was transferred to the mass spectrometer and ionized by heated electrospray negative ionization (HESI) and the analyte was quantified using a six-point calibration curve. The validated analytical measurement range (AMR) is 30-1,000 nMol/L. Dilutions of 10 and 200-fold were validated giving a clinical reportable range (CRR) of 30-200,000 nMol/L. The between-day and within-day imprecision va.. View More»
  DOI: 10.4172/2157-7064.1000336

  Abstract PDF

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