Journal of Agricultural Science and Food Research
Open Access

Ayele YZ

Publications

• Research Article
  Low Cost Sterilization Technique and In Vitro Initiation of Vanilla (Vanilla planifolia Andr.)
  Author(s): Ayele YZ and Tefera WAyele YZ and Tefera W
  
  Microbial contamination is one of the most serious problems of plant tissue culture. Treating the mother plant withchemicals and nourishing them in a greenhouse instead of letting them to laboratory directly from the field hasgreater importance to plant tissue culture techniques to reduce contamination los and to increase potency level. Inthe present study, effort was made to develop an effective surface sterilization and shoot imitation protocol with anenhanced survival rate of nodal explants of vanilla by treating with various sterilant combination with differentexposure time and hormonal combinations to enhance initiation. The explants were collected from JimmaAgricultural Research Center greenhouse and used for the subsequent experiments. In all cases, the experimentswere laid out in Completely Randomized Design (CRD) with factorial treatment combinations and replicated threetimes.. View More»
  

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