Biochemistry & Pharmacology: Open Access
Open Access

Barry Morris

Publications

• Research Article
  The Effects of Gold and Silver Nanoparticles on an Enzymatic Reaction Between Horseradish Peroxidase and 3,3',5,5'-Tetramethylbenzidine
  Author(s): Barry Morris and Farhad Behzad Barry Morris and Farhad Behzad
  
  The purpose of this investigation was to study the effects of gold (Au) and silver (Ag) nanoparticles on a commonly used enzymatic reaction between horseradish peroxidase (HRP) and the substrate, 3,3',5,5'-tetramethylbenzidine (TMB). Two different methodologies were used in this study. The first was the addition of small quantities of nanoparticles, directly onto a solution of streptavidin-peroxidase (streptavidin bound to HRP), prior to its reaction with TMB. The second was the addition of nanoparticles to immobilized streptavidin-peroxidase, covalently bounded to wells of a microtitre plate. In both cases, reactions with TMB were measured by visible absorbance spectroscopy, using a microtitre plate reader. The results indicate that both gold and silver nanoparticles have a significant effect; with gold suppressing the reaction and silver enhancing it. .. View More»
  DOI: 10.4172/2167-0501.1000146

  Abstract PDF

Relevant Topics