Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access

Bianca Malecki

Publications

• Research Article
  Nuclear Routing Networks Span between Nuclear Pore Complexes and Genomic DNA to Guide Nucleoplasmic Trafficking of Biomolecules
  Author(s): Marek Malecki, Bianca MaleckiMarek Malecki, Bianca Malecki
  
  In health and disease, biomolecules, which are involved in gene expression, recombination, or reprogramming have to traffic through the nucleoplasm, between Nuclear Pore Complexes (NPCs) and genomic DNA (gDNA). This trafficking is guided by the recently revealed Nuclear Routing Networks (NRNs). In this study, we aimed to investigate, if the NRNs have established associations with the genomic DNA in situ and if the NRNs have capabilities to bind the DNA de novo. Moreover, we aimed to study further, if nucleoplasmic trafficking of the histones, rRNA, and transgenes’ vectors, between the NPCs and gDNA, is guided by the NRNs. We used Xenopus laevis oocytes as the model system. We engineered the transgenes’ DNA vectors equipped with the SV40 LTA Nuclear Localization Signals (NLS) and/or HIV Rev Nuclear Export Signals (NES). We purified histones, 5S rRNA, and gDNA... View More»
  

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