Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Brett A Chromy
Department of Pathology and Laboratory Medicine, School of Medicine,
Sacramento, CA 95817
Tanzania
Research Article
Multivariate Statistical Analysis of Diverse Strains of Yersinia pestis by Comparative Proteomics
Author(s): Todd H Corzett, Angela M Eldridge, Jennifer S Knaack, Christopher H Corzett, Sandra L McCutchen-Maloney and Brett A ChromyTodd H Corzett, Angela M Eldridge, Jennifer S Knaack, Christopher H Corzett, Sandra L McCutchen-Maloney and Brett A Chromy
To address the difficulty in characterizing unusual, engineered or emergent pathogens in clinical and environmental samples, novel methods to discover proteins that differentiate pathogenic strains are needed. Differentially expressed proteins that reveal the function of an uncharacterized strain of bacteria can be considered biomarkers; panels of these can lead to improved pathogen classification and characterization. To this end, the protein expression patterns of differentially virulent isolates of the plague pathogen, Yersinia pestis, were studied using two-dimensional difference gel electrophoresis (2-D DIGE). The resulting characterization was used to identify a protein expression panel for the clustering and classification of Y. pestis strains. Two different methods were used to produce different biomarker panels based on either experimental- or pattern-based clustering. Each p.. View More»
DOI:
10.4172/jpb.1000282