Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Bruce A. Bunnell
Tanzania
Research Article
Differentiation of Human Adipose-derived Stem Cells along the Keratocyte Lineage In vitro
Author(s): Shijia Zhang, Ladan Espandar, Kathleen M.P. Imhof, and Bruce A. BunnellShijia Zhang, Ladan Espandar, Kathleen M.P. Imhof, and Bruce A. Bunnell
Purpose: To evaluate differentiation of human adipose-derived stem cells (hASCs) to the keratocyte lineage by co-culture with primary keratocytes in vitro.Materials and Methods: A co-culture system using transwell inserts to grow hASCs on bottom and keratocytes on top in keratocyte differentiating medium (KDM) was developed. hASCs that were cultured in complete culture medium (CCM) and KDM were used as control. After 16 days, hASCs were examined for morphologic changes and proliferation by cell count. qRT-PCR and flow cytometry were used to detect the expression of aldehyde dehydrogenase 3 family, member A1 (ALDH3A1) and keratocan.Results: hASCs became more dendritic and elongated in co-culture system relative to CCM and KDM. The doubling time of the cells was longer as differentiation progressed. qRT-PCR showed a.. View More»
DOI:
10.4172/2155-9570.1000270