Advances in dairy Research
Open Access

Carlo G Zambonin

Publications

• Research Article
  MALDI-Q-TOF-MS Ionization and Fragmentation of Phospholipids and Neutral Lipids of Dairy Interest Using Variable Doping Salts
  Author(s): Cosima D Calvano and Carlo G Zambonin Cosima D Calvano and Carlo G Zambonin
  
  This paper reports the potential of matrix assisted laser desorption ionization quadrupole-time-of-flight (MALDI-Q-TOF) mass spectrometry (MS) for the analysis of lipids of dairy interest, i.e., glycerophospholipids (Phosphatidylcholine, Phosphatidylethanolamine, Phosphatidylinositol), sphingolipids (Sphingomyelin) and glycerolipids (Triacylglycerols, Diacylglycerols), respectively. The matrix used was 2,5-dihydroxybenzoic acid (DHB), together with cationizing agents such as sodium and lithium salt that enhanced fragmentation of most lipid classes. The alkaline adducts obtained helped to define in a fast way the polar head groups of different glycerophospholipid classes and provided information about the constituent fatty acid residues. Moreover, information about the position (sn-1, and -2) of the fatty acid residue on the glycerol backbone was obtained. Besides, lithiu.. View More»
  DOI: 10.4172/2329-888X.1000101

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