Journal of Proteomics & Bioinformatics
Open Access

Carol B Fowler

Publications

• Research Article
  Improving the Proteomic Analysis of Archival Tissue by Using Pressure- Assisted Protein Extraction: A Mechanistic Approach
  Author(s): Carol B Fowler, Timothy J O'Leary and Jeffrey T MasonCarol B Fowler, Timothy J O'Leary and Jeffrey T Mason
  
  Formaldehyde-fixed, paraffin-embedded (FFPE) tissue repositories represent a valuable resource for the retrospective study of disease progression and response to therapy. However, the proteomic analysis of FFPE tissues has been hampered by formaldehyde-induced protein modifications, which reduce protein extraction efficiency and may lead to protein misidentification. Here, we demonstrate the use of heat augmented with high hydrostatic pressure (40,000 psi) as a novel method for the recovery of intact proteins from FFPE tissue. Our laboratory has taken a mechanistic approach to developing improved protein extraction protocols, by first studying the reactions of formaldehyde with proteins and ways to reverse these reactions, then applying this approach to a model system called a “tissue surrogate”, which is a gel formed by treating high concentrations of cytoplasmic proteins.. View More»
  DOI: 10.4172/jpb.1000315

  Abstract PDF

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