Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Chao-Jen Kuo
People's Republic of China
Research Article
Quantitative Proteomics Analysis of Differentially Expressed Proteins Involved in Renal Clear Cell Carcinoma by Shotgun Approach Coupled with Stable Isotope Dimethyl Labeling
Author(s): Shih-Shin Liang, Chao-Jen Kuo, Shu-Wen Chi, Wen-Jeng Wu, Shui-Tein Chen and Shyh-Horng ChiouShih-Shin Liang, Chao-Jen Kuo, Shu-Wen Chi, Wen-Jeng Wu, Shui-Tein Chen and Shyh-Horng Chiou
Renal clear cell carcinoma (RCC) when detected incidentally by ultrasonic imaging generally shows an advanced disease stage of being diagnosed metastatic as a result of lacking specific biomarkers at an early stage. To date, RCC is still ranked as the most common renal cancer and considered as one of the most treatment-resistant metastatic malignancies. In this study, a quantitative proteomics approach by means of gel-free shotgun proteomics methodology and stable isotope dimethyl labeling coupled with nano-liquid chromatography/ tandem mass (nanoLCMS/ MS) have been employed to identify 18 up-regulated and 48 down-regulated proteins in RCC samples. It is worth noting that binding and structural proteins in renal clear cells accounted for 43% and 33% of up-regulated proteins respectively, while catalytic enzymes occupied as high as 73% of down-regulated proteins. Collectively, instead .. View More»
DOI:
10.4172/jpb.S7-003